Chemometric assisted UV spectrophotometric method and RP-HPLC was developed for simultaneous estimation of metoprolol succinate (METO) and Olmesartanmedoxomil (OLME) in their combined dosage form. Multivariate calibration methods (Classical Least Squares and Inverse Least Squares) using the absorption spectra in the region of 220-300 nm were proposed for the resolution of strongly overlapping spectra of metoprolol succinate (METO) and olmesartan medoxomil (OLME) in binary mixtures. In RP-HPLC, the method was developed using Phenomenex Luna C18 column (150 X 4.6 mm i.d., 5μm), with mobile phase composition of acetonitrile:phosphate buffer, pH 3.0 (45:55, v/v). The flow rate was 0.7 mL/min and effluent was monitored at 224 nm. The retention time of METO and OLME were 2.408 min and 6.167 min, respectively. Linearity was observed over the concentration range of 5-30 μg/ml for both the drugs. The developed method was validated as per ICH guidelines. The proposed methods were successfully applied for the quantification of METO and OLME in pharmaceutical formulations.
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